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ATCC panc10 05
Panc10 05, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 207 article reviews

panc10 05 - by Bioz Stars, 2026-04

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( A ) Schematic representation of the microfluidic system used to study the interactions between pancreatic ductal adenocarcinoma (PDAC) cells and endothelial cells. ( B – D ) Labeled (CellTrace) PDAC cells were perfused over an endothelial monolayer at 400 µm/s for 10 min. Samples were fixed, stained with phalloidin and DAPI, and imaged using a spinning disk confocal microscope. ( B ) A representative image shows the endothelial monolayer with attached AsPC-1 cells. The red box highlights a region of interest that is magnified. Scale bars: 100 µm (main), 30 µm (inset). ( C ) The number of attached cells was quantified for each cell line. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 25–36 fields of view, 3–7 biological repeats). The P values were determined using a randomization test. ( D ) Frequency plot showing the overall distribution of cancer cells across the microfluidic channel from the edge (border) to the center ( n = 302–2279 cells) and highlighting the region selected for subsequent live-cell imaging. ( E – L ) AsPC-1, MIA PaCa-2, and Panc 10.05 cells were perfused over an endothelial monolayer and imaged using a brightfield microscope at 25 Hz for 8 min, with flow speeds varied at 2-min intervals: 400 µm/s (High, H), 200 µm/s (Medium, M), and 100 µm/s (Low, L). Each sequence concluded with an increased flow speed of 400 µm/s to test the stability of the adhered cells (Wash, W). ( F ) A representative brightfield image (BF) showing the detected PDAC cells at two different time points and the resulting tracks. ( G ) The number of arrested PDAC cells over time for each cell line tested. Bold lines indicate the average, and shaded areas represent the SD (4–7 biological repeats, see “Methods”). The different flow speeds are shown. ( H ) The attachment rate for each cell line at each flow speed is displayed as a bar chart (mean +/− SEM) with individual data points (4–7 biological repeats, see “Methods”). The P values were determined using a randomization test. ( I ) Percentage of AsPC-1 and MIA PaCa-2 cells arresting as single cells per movie. Here, a cluster is defined as at least two cells that arrest together (at the same time) within a cell diameter of each other (see “Methods” for details). Results from the various flow speeds are pooled ( n > 24 videos, 6–7 biological repeats; see “Methods”). ( J – L ) Analysis of track metrics for MIA PaCa-2, AsPC-1, and Panc 10.05 cells at different flow speeds. ( J ) Plot of mean, maximum, and minimum track speeds for each cell line. Data are presented as mean ± SD. ( K ) Total distance traveled by PDAC cells during perfusion. ( L ) Forward Migration Index (FMI) along the flow direction for each cell line. ( I , K , L ) Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points outside the whiskers are depicted as individual dots ( n = 3011–8227 tracks, 4–7 biological repeats, see “Methods”). The numerical data and images used for this figure, as well as statistical summaries including pairwise Cohen’s d values and results from statistical tests, have been archived on Zenodo (10.5281/zenodo.17232437).

Journal: The EMBO Journal

Article Title: Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers

doi: 10.1038/s44318-025-00678-9

Figure Lengend Snippet: ( A ) Schematic representation of the microfluidic system used to study the interactions between pancreatic ductal adenocarcinoma (PDAC) cells and endothelial cells. ( B – D ) Labeled (CellTrace) PDAC cells were perfused over an endothelial monolayer at 400 µm/s for 10 min. Samples were fixed, stained with phalloidin and DAPI, and imaged using a spinning disk confocal microscope. ( B ) A representative image shows the endothelial monolayer with attached AsPC-1 cells. The red box highlights a region of interest that is magnified. Scale bars: 100 µm (main), 30 µm (inset). ( C ) The number of attached cells was quantified for each cell line. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 25–36 fields of view, 3–7 biological repeats). The P values were determined using a randomization test. ( D ) Frequency plot showing the overall distribution of cancer cells across the microfluidic channel from the edge (border) to the center ( n = 302–2279 cells) and highlighting the region selected for subsequent live-cell imaging. ( E – L ) AsPC-1, MIA PaCa-2, and Panc 10.05 cells were perfused over an endothelial monolayer and imaged using a brightfield microscope at 25 Hz for 8 min, with flow speeds varied at 2-min intervals: 400 µm/s (High, H), 200 µm/s (Medium, M), and 100 µm/s (Low, L). Each sequence concluded with an increased flow speed of 400 µm/s to test the stability of the adhered cells (Wash, W). ( F ) A representative brightfield image (BF) showing the detected PDAC cells at two different time points and the resulting tracks. ( G ) The number of arrested PDAC cells over time for each cell line tested. Bold lines indicate the average, and shaded areas represent the SD (4–7 biological repeats, see “Methods”). The different flow speeds are shown. ( H ) The attachment rate for each cell line at each flow speed is displayed as a bar chart (mean +/− SEM) with individual data points (4–7 biological repeats, see “Methods”). The P values were determined using a randomization test. ( I ) Percentage of AsPC-1 and MIA PaCa-2 cells arresting as single cells per movie. Here, a cluster is defined as at least two cells that arrest together (at the same time) within a cell diameter of each other (see “Methods” for details). Results from the various flow speeds are pooled ( n > 24 videos, 6–7 biological repeats; see “Methods”). ( J – L ) Analysis of track metrics for MIA PaCa-2, AsPC-1, and Panc 10.05 cells at different flow speeds. ( J ) Plot of mean, maximum, and minimum track speeds for each cell line. Data are presented as mean ± SD. ( K ) Total distance traveled by PDAC cells during perfusion. ( L ) Forward Migration Index (FMI) along the flow direction for each cell line. ( I , K , L ) Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points outside the whiskers are depicted as individual dots ( n = 3011–8227 tracks, 4–7 biological repeats, see “Methods”). The numerical data and images used for this figure, as well as statistical summaries including pairwise Cohen’s d values and results from statistical tests, have been archived on Zenodo (10.5281/zenodo.17232437).

Article Snippet: Panc 10.05 cells ( H. sapiens ) , ATCC , ATCC-CRL-2547.

Techniques: Labeling, Staining, Microscopy, Live Cell Imaging, Sequencing, Migration

( A , B ) Adhesion of labeled PDAC cell lines to an endothelial monolayer without flow. Cells were fixed, stained with phalloidin and DAPI, and imaged using a spinning disk confocal microscope. ( A ) A representative image of the endothelial monolayer with attached PDAC cells. Scale bar: 100 µm. ( B ) The number of attached cells was quantified for each cell line. Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 10–27 fields of view, 2–9 biological repeats). The P values were determined using a randomization test. MIA PaCa-2 vs AsPC-1, P value = 0.0001. MIA PaCa-2/SW1990/AsPC-1 (grouped on graph) vs Panc 10.05, P value = 0.0001. ( C ) Size distribution of PDAC cell lines. Cells in suspension were imaged using a brightfield microscope, and their diameters were manually measured using Fiji. Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 39–40 cells). The P values were determined using a randomization test. Non-significant comparisons not shown. AsPC-1 vs MIA PaCa-2, P value = 0.0001. MIA PaCa-2 vs Panc 10.05, P value = 0.0001. BxPC-3 vs Panc 10.05, P value = 0.0001. ( D , E ) Endothelial monolayers, either untreated or treated with IL-1β (10 ng/ml for 2 h and 5 ng/ml for 16 h), were fixed and stained to visualize E-selectin, VCAM1, ICAM-1, and CD44. Stainings were performed without permeabilization to specifically label surface-accessible adhesion molecules. Images were captured using a spinning disk confocal microscope. ( D ) Representative fields of view are shown. Scale bar: 100 µm. ( E ) Quantification of the marker per field of view is presented. Intensities were normalized to the number of nuclei per field of view, as well as the average intensity measured in the control in each repeat. Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 45 field of view, 3 biological repeats). The P values were determined using a randomization test. E-selectin, Ctrl vs IL-1β 2 h, P value = 0.0001 Ctrl vs IL-1β 16 h, P value = 0.0001. VCAM-1, Ctrl vs IL-1β 2 h, P value = 0.0001 Ctrl vs IL-1β 16 h, P value = 0.0001. ICAM-1, Ctrl vs IL-1β 2 h, P value = 0.0001 Ctrl vs IL-1β 16 h, P value = 0.0001. CD44, Ctrl vs IL-1β 16 h, P value = 0.0001. ( F , G ) The number of arrested neutrophils ( F ) or PBMCs ( G ) over time, in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h and 5 ng/ml for 16 h). Bold lines indicate the average, and shaded areas represent the SD (4–7 biological repeats, see “Methods”). ( H ) The number of arrested PDAC cells over time for each cell line tested, with (2 h) and without IL-1β stimulation (PDAC Ctrl results were already displayed in Fig. ). Bold lines indicate the average, and shaded areas represent the SD (2–7 biological repeats, see “Methods”). ( I ) The number of arrested PDAC and immune cells over time without IL-1β stimulation (PDAC Ctrl results were already displayed in Fig. ). Bold lines indicate the average, and shaded areas represent the SD (3–8 biological repeats, see “Methods”). The numerical data and images used for this figure, as well as statistical summaries including pairwise Cohen’s d values and results from statistical tests, have been archived on Zenodo (https://doi.org/10.5281/zenodo.17232437).

Journal: The EMBO Journal

Article Title: Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers

doi: 10.1038/s44318-025-00678-9

Figure Lengend Snippet: ( A , B ) Adhesion of labeled PDAC cell lines to an endothelial monolayer without flow. Cells were fixed, stained with phalloidin and DAPI, and imaged using a spinning disk confocal microscope. ( A ) A representative image of the endothelial monolayer with attached PDAC cells. Scale bar: 100 µm. ( B ) The number of attached cells was quantified for each cell line. Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 10–27 fields of view, 2–9 biological repeats). The P values were determined using a randomization test. MIA PaCa-2 vs AsPC-1, P value = 0.0001. MIA PaCa-2/SW1990/AsPC-1 (grouped on graph) vs Panc 10.05, P value = 0.0001. ( C ) Size distribution of PDAC cell lines. Cells in suspension were imaged using a brightfield microscope, and their diameters were manually measured using Fiji. Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 39–40 cells). The P values were determined using a randomization test. Non-significant comparisons not shown. AsPC-1 vs MIA PaCa-2, P value = 0.0001. MIA PaCa-2 vs Panc 10.05, P value = 0.0001. BxPC-3 vs Panc 10.05, P value = 0.0001. ( D , E ) Endothelial monolayers, either untreated or treated with IL-1β (10 ng/ml for 2 h and 5 ng/ml for 16 h), were fixed and stained to visualize E-selectin, VCAM1, ICAM-1, and CD44. Stainings were performed without permeabilization to specifically label surface-accessible adhesion molecules. Images were captured using a spinning disk confocal microscope. ( D ) Representative fields of view are shown. Scale bar: 100 µm. ( E ) Quantification of the marker per field of view is presented. Intensities were normalized to the number of nuclei per field of view, as well as the average intensity measured in the control in each repeat. Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points falling outside the whiskers are depicted as individual dots ( n = 45 field of view, 3 biological repeats). The P values were determined using a randomization test. E-selectin, Ctrl vs IL-1β 2 h, P value = 0.0001 Ctrl vs IL-1β 16 h, P value = 0.0001. VCAM-1, Ctrl vs IL-1β 2 h, P value = 0.0001 Ctrl vs IL-1β 16 h, P value = 0.0001. ICAM-1, Ctrl vs IL-1β 2 h, P value = 0.0001 Ctrl vs IL-1β 16 h, P value = 0.0001. CD44, Ctrl vs IL-1β 16 h, P value = 0.0001. ( F , G ) The number of arrested neutrophils ( F ) or PBMCs ( G ) over time, in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h and 5 ng/ml for 16 h). Bold lines indicate the average, and shaded areas represent the SD (4–7 biological repeats, see “Methods”). ( H ) The number of arrested PDAC cells over time for each cell line tested, with (2 h) and without IL-1β stimulation (PDAC Ctrl results were already displayed in Fig. ). Bold lines indicate the average, and shaded areas represent the SD (2–7 biological repeats, see “Methods”). ( I ) The number of arrested PDAC and immune cells over time without IL-1β stimulation (PDAC Ctrl results were already displayed in Fig. ). Bold lines indicate the average, and shaded areas represent the SD (3–8 biological repeats, see “Methods”). The numerical data and images used for this figure, as well as statistical summaries including pairwise Cohen’s d values and results from statistical tests, have been archived on Zenodo (https://doi.org/10.5281/zenodo.17232437).

Article Snippet: Panc 10.05 cells ( H. sapiens ) , ATCC , ATCC-CRL-2547.

Techniques: Labeling, Staining, Microscopy, Suspension, Marker, Control

( A – M ) Neutrophils, PBMCs, AsPC-1, MIA PaCa-2, and Panc 10.05 cells were perfused over an endothelial monolayer in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h) and imaged using a brightfield microscope at 25 Hz for 8 min. Flow speeds varied in 2-min intervals: 400 µm/s (High, H), 200 µm/s (Medium, M), 100 µm/s (Low, L), and 400 µm/s (Wash, W). ( A – H ) Analysis of neutrophils and PBMCs attachment to the endothelial monolayer in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h of pretreatment). ( A , B ) Representative brightfield images showing neutrophils and PBMCs and their resulting tracks. ( C ) The number of arrested Neutrophils (Neutro.) and PBMCs over time, with bold lines indicating the average and shaded areas representing the SD (4–8 biological repeats, see “Methods”). ( D ) The attachment rates for neutrophils and PBMCs at each flow speed are displayed as a bar chart (mean ± SEM) with individual data points (4–8 biological repeats, see “Methods”). ( E ) Percentage of neutrophils and PBMCs cells arresting as single cells per movie in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h). Here, a cluster is defined as at least two cells that arrest together (at the same time) within a cell diameter of each other (see “Methods” for details). Results from the various flow speeds are pooled ( n > 16 videos, 4–8 biological repeats; see “Methods”). ( F – H ) Analysis of track metrics for neutrophils and PBMCs at different flow speeds. ( F ) Plot of mean, maximum, and minimum track speeds. Data are presented as mean ± SD (4–8 biological repeats, see “Methods”). ( G ) Total distance traveled by neutrophils and PBMCs during perfusion. ( H ) FMI along the flow direction for each condition. ( E , G , H ) Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points outside the whiskers are depicted as individual dots ( n = 3044–12,905 tracks, 4–8 biological repeats, see “Methods”). ( I – K ) Impact of IL-1β stimulation (10 ng/ml for 2 h) on PDAC cell attachment to the endothelial monolayer. The number of arrested MIA PaCa-2 ( I ), AsPC-1 ( J ), and Panc 10.05 ( K ) cells over time, with bold lines indicating the average and shaded areas representing the standard deviation (2–7 biological repeats, see “Methods”). Different flow speeds are shown. ( L , M ) Comparison of PDAC and immune cells’ attachment to endothelial cells in the presence of IL-1β stimulation (10 ng/ml for 2 h). The number of arrested cells over time, with bold lines indicating the average and shaded areas representing the standard deviation (2–8 biological repeats, see “Methods”). ( M ) The attachment rate for each condition is displayed as a bar chart (mean ± SEM) with individual data points. The numerical data and images used for this figure, as well as statistical summaries including pairwise Cohen’s d values and results from statistical tests, have been archived on Zenodo (10.5281/zenodo.17232437).

Journal: The EMBO Journal

Article Title: Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers

doi: 10.1038/s44318-025-00678-9

Figure Lengend Snippet: ( A – M ) Neutrophils, PBMCs, AsPC-1, MIA PaCa-2, and Panc 10.05 cells were perfused over an endothelial monolayer in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h) and imaged using a brightfield microscope at 25 Hz for 8 min. Flow speeds varied in 2-min intervals: 400 µm/s (High, H), 200 µm/s (Medium, M), 100 µm/s (Low, L), and 400 µm/s (Wash, W). ( A – H ) Analysis of neutrophils and PBMCs attachment to the endothelial monolayer in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h of pretreatment). ( A , B ) Representative brightfield images showing neutrophils and PBMCs and their resulting tracks. ( C ) The number of arrested Neutrophils (Neutro.) and PBMCs over time, with bold lines indicating the average and shaded areas representing the SD (4–8 biological repeats, see “Methods”). ( D ) The attachment rates for neutrophils and PBMCs at each flow speed are displayed as a bar chart (mean ± SEM) with individual data points (4–8 biological repeats, see “Methods”). ( E ) Percentage of neutrophils and PBMCs cells arresting as single cells per movie in the presence or absence of IL-1β stimulation (10 ng/ml for 2 h). Here, a cluster is defined as at least two cells that arrest together (at the same time) within a cell diameter of each other (see “Methods” for details). Results from the various flow speeds are pooled ( n > 16 videos, 4–8 biological repeats; see “Methods”). ( F – H ) Analysis of track metrics for neutrophils and PBMCs at different flow speeds. ( F ) Plot of mean, maximum, and minimum track speeds. Data are presented as mean ± SD (4–8 biological repeats, see “Methods”). ( G ) Total distance traveled by neutrophils and PBMCs during perfusion. ( H ) FMI along the flow direction for each condition. ( E , G , H ) Results are presented as boxplots, where the whiskers extend from the 10th to the 90th percentiles. The boxes capture the interquartile range, with the median marked by a line within each box. Data points outside the whiskers are depicted as individual dots ( n = 3044–12,905 tracks, 4–8 biological repeats, see “Methods”). ( I – K ) Impact of IL-1β stimulation (10 ng/ml for 2 h) on PDAC cell attachment to the endothelial monolayer. The number of arrested MIA PaCa-2 ( I ), AsPC-1 ( J ), and Panc 10.05 ( K ) cells over time, with bold lines indicating the average and shaded areas representing the standard deviation (2–7 biological repeats, see “Methods”). Different flow speeds are shown. ( L , M ) Comparison of PDAC and immune cells’ attachment to endothelial cells in the presence of IL-1β stimulation (10 ng/ml for 2 h). The number of arrested cells over time, with bold lines indicating the average and shaded areas representing the standard deviation (2–8 biological repeats, see “Methods”). ( M ) The attachment rate for each condition is displayed as a bar chart (mean ± SEM) with individual data points. The numerical data and images used for this figure, as well as statistical summaries including pairwise Cohen’s d values and results from statistical tests, have been archived on Zenodo (10.5281/zenodo.17232437).

Article Snippet: Panc 10.05 cells ( H. sapiens ) , ATCC , ATCC-CRL-2547.

Techniques: Microscopy, Cell Attachment Assay, Standard Deviation, Comparison

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